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1492r primers  (Elim Bio)
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universal primers 27 f and 1492r  (Metabion International AG)
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Metabion International AG universal primers 27 f and 1492r
Gel electrophoresis of 16 S rRNA gene PCR products of endophytic actinobacteria isolates, amplified using 27 F (5’-AGAGTTTGATCMTGGCTCAG-3’) and <t>1492R</t> (5’-TACGGGTACCTTGTTACGACTT-3’) primers. The 1.5 kb amplified 16 S rRNA gene fragments were visualized on a 1.2% agarose gel. Lanes, from left to right: 100–3000 bp ladder (lane 1), KUMS-B9 (lane 2), KUMS-B10 (lane 3), KUMS-B11 (lane 4), KUMS-B12 (lane 5), KUMS-B16 (lane 6), KUMS-B13 (lane 7), KUMS-B14 (lane 8), KUMS-B15 (lane 9), and IKBG04 (lane 10)
Universal Primers 27 F And 1492r, supplied by Metabion International AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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universal primers 27 f and 1492r - by Bioz Stars, 2026-07
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Gel electrophoresis of 16 S rRNA gene PCR products of endophytic actinobacteria isolates, amplified using 27 F (5’-AGAGTTTGATCMTGGCTCAG-3’) and 1492R (5’-TACGGGTACCTTGTTACGACTT-3’) primers. The 1.5 kb amplified 16 S rRNA gene fragments were visualized on a 1.2% agarose gel. Lanes, from left to right: 100–3000 bp ladder (lane 1), KUMS-B9 (lane 2), KUMS-B10 (lane 3), KUMS-B11 (lane 4), KUMS-B12 (lane 5), KUMS-B16 (lane 6), KUMS-B13 (lane 7), KUMS-B14 (lane 8), KUMS-B15 (lane 9), and IKBG04 (lane 10)

Journal: BMC Microbiology

Article Title: Endophytic actinobacteria from Mentha longifolia and Lonicera nummulariifolia : a novel source against antibiotic resistance

doi: 10.1186/s12866-025-04089-y

Figure Lengend Snippet: Gel electrophoresis of 16 S rRNA gene PCR products of endophytic actinobacteria isolates, amplified using 27 F (5’-AGAGTTTGATCMTGGCTCAG-3’) and 1492R (5’-TACGGGTACCTTGTTACGACTT-3’) primers. The 1.5 kb amplified 16 S rRNA gene fragments were visualized on a 1.2% agarose gel. Lanes, from left to right: 100–3000 bp ladder (lane 1), KUMS-B9 (lane 2), KUMS-B10 (lane 3), KUMS-B11 (lane 4), KUMS-B12 (lane 5), KUMS-B16 (lane 6), KUMS-B13 (lane 7), KUMS-B14 (lane 8), KUMS-B15 (lane 9), and IKBG04 (lane 10)

Article Snippet: Polymerase chain reaction (PCR) of 16S rRNA gene was performed for molecular identification of strains using two universal primers (synthesized by Metabion, Germany) 27 F (5’-AGAGTTTGATCMTGGCTCAG − 3’), and 1492R (5’-TACGGGTACCTTGTTACGACTT-3’) [ , ].

Techniques: Nucleic Acid Electrophoresis, Amplification, Agarose Gel Electrophoresis